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Published on:January 2016
RGUHS Journal of Pharmaceutical Sciences, 2016; 6(1):12-19
Research Article | doi:10.5530/rjps.2016.1.3

Cardioprotective Effect of Rosa centifolia against Doxorubicin-induced Myocardial Toxicity in Albino Rats

Authors and affiliation (s):

S K Nimbal and B C Koti*

Department of Pharmacology, KLE University’s College of Pharmacy, Vidyanagar, Hubballi-580031, Karnataka, INDIA.


Background: The Anthracycline derivative, doxorubicin is a cytotoxic agent with proven efficacy in various malignancies. The clinical usefulness has been limited due to its cardiac toxicity. Objectives: To study the influence of ethanolic extract of petals of Rosa centifolia (EERC) against doxorubicin (Dox) induced myocardial toxicity in rats. Methods: Cardiotoxicity was produced by cumulative administration of Dox (15 mg/kg for two weeks, i.p.). EERC (100 and 200 mg/kg, p.o) was administered as pretreatment for two weeks followed by Dox on alternative days for two weeks. The parameters such as, biomarker enzymes like Creatine kinase (CK-MB), lactate dehydrogenase (LDH) and cardiac Troponin-I (cTnI), biochemical parameters like alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), antioxidant enzymes like superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) and lipid peroxidation were monitored after three weeks of last dose. Heart histopathalogical studies were also carried out to evaluate myocardial toxicity. Results: The repeated administration of Dox-induced cardiomyopathy was characterized by decrease of antioxidant enzymes and elevated levels of cardiac biomarkers. Pretreatment with the EERC (200 mg/kg) significantly protected heart from the toxic effects of doxorubicin by decreasing the elevated level of biomarker enzymes like LDH, CK-MB and absence of cardiac troponin-I and biochemical parameters such as ALT, AST, and ALP back to normal. The EERC increased the reduced level of GSH, SOD and CAT while decreased the elevated level of malondialdehyde (MDA) in cardiac tissue along with normalizing the histological architecture of heart tissue. Conclusion: The biochemical and histopathological data evidently substantiate the cardioprotective effect of EERC, which could be attributed to its antioxidant property.

Key words: Antioxidant, Cardiotoxicity, Rosa centifolia, Doxorubicin.



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